Synthetic Biology
Systems Biology

Designing synthetic DNA molecules.
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Synthetic Biology
Systems Biology

Keeping synthetic biology safe.
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Synthetic Biology
Systems Biology

Optimizing gene synthesis
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Synthetic Biology
Systems Biology

Measuring gene expression in single cells.
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Synthetic Biology
Systems Biology

Play the DNA factory game
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Toward a more efficient photosynthesis

A team lead by Maureen Hanson (Cornell University) has successfully replaced the native RUBISCO enzyme of tobacco cells with a faster RUBISCO of cyanobacterial origin. The RUBISCO enzyme is part of the photosynthetic pathway. It converts CO2 into sugar. Because of the complexity of this enzyme, this paper is a major landmark of plant biotechnology. […]

GenoSIGHT paper is published in PLoS ONE

GenoSIGHT

After several years of incubation and internal validation, we are pleased to announce the publication of an article describing GenoSIGHT, an adaptive cytometry system, developed with a grant from the National Science Foundation (NSF Award#0963988).  GenoSIGHT is an “intelligent” system capable of adapting the image acquisition process to the dynamics of the cells under observation. […]

PL-501-002: Poly-L-lysine coated coverslips

Revision number: 1 Prepared on: 04/06/14 Reviewed by: Jean Peccoud Reviewed on: 09/02/14 Purpose Describes the protocol for coating coverslips with poly-L-lysine to allow yeast cells to stick Scope This SOP is primarily used to make coverslips for use in single-mRNA FISH imaging experiments with  budding yeast (Saccharomyces cerevisiae). However, it could also be used with […]

PL-500-001: Metal Halide Bulb Replacement

Revision number: 1 Prepared on: 08/30/14 Reviewed by: Jean Peccoud Reviewed on: 09/02/14 Purpose This SOP describes how to change the Metal Halide bulb in the Lumen Dynamics X-Cite 120PQ lamp attached to the Zeiss Axio Observer. Each bulb has a lifetime of ~2000 hours. Scope This SOP is relevant for anyone using the Zeiss […]

PL-004-002: Purchasing general laboratory supplies

Revision number: 1 Prepared on: 08/01/14 Reviewed by: Jean Peccoud Reviewed on: 08/26/14 Purpose This SOP describes the process of placing orders for general supplies (reagents, chemicals, consumables, etc.) through HokieMart. Scope The purchase of laboratory supplies takes place both in HokieMart, the university purchasing system, and in LabCollector. Because, all VBI orders are placed […]

PL-004-001: Requesting supplies through LabCollector

PL-004-001: Requesting supplies through LabCollector

Revision number:1 Prepared on: 07/31/14 Reviewed by: Jean Peccoud Reviewed on: 08/26/14 Purpose This SOP describes the process of using the LabCollector order list to request the purchase of laboratory supplies. Scope Lab supplies are purchased through Hokiemart. VT only allows Hokiemart access to 2 people per lab (including the PI). Because of this limitation, […]

PL-004-003: Purchasing oligonucleotides in 96-well plates

Revision number: 1 Prepared on: 08/01/14 Reviewed by: Jean Peccoud Reviewed on: 08/26/14 Purpose This SOP describes the process of requesting and ordering oligos in 96-well format Scope We use oligos for sequencing of existing plasmids and fabrication of new plasmids. IDT is our preferred vendor for ordering oligonucleotides. We have negotiated a special quote […]

Gene Synthesis Course September 6-11, 2014

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Gene synthesis technologies are rapidly changing research paradigms in molecular biology. The purpose of this course is to provide students a practical understanding of gene synthesis so that they can make the most of it and better interact with providers of gene synthesis services. Background The development of recombinant DNA technology revolutionized biology, allowing researchers […]

PL-101-004: Creating monthly OMERO report

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Revision number: Prepared on: 07/30/14 Reviewed by: Jean Peccoud Reviewed on: 08/21/14 Purpose This SOP describes how to create the plot of OMERO activity that should be completed on a monthly basis. Scope The report that is generated will be displayed on the white board outside of the PI’s office along with the other monthly […]